Today, the cryogenic storage systems market is very competitive and all companies advertise their solutions as innovative. In cryogenic storage of biological materials, temperatures below – 130 °C are usually required.

However, they are all similar to each other and are based on one technology, cooling directly via liquid nitrogen. Liquid nitrogen is cheap and abundant, due to which it became the basis of the cryogenic storage.

Liquid nitrogen-based cooling may be extremely useful for areas where electricity supply is not stable and processes in which temperature uniformity and the risk of contamination are not prioritized. However, there are many difficulties associated with using liquid nitrogen for cooling of biomedical samples or using it in industrial processes.

1/ Transporting liquid nitrogen from the production site to the site where it is used may be difficult and expensive if the LN2 production site is located far away.

2/ Storage of liquid nitrogen can be furthermore problematic as it tends to evaporate over time, and if the tanks in which they are stored have faulty pressure valves, it is ticking time bomb, as there is a large risk of explosion.

3/ Rooms in which numerous liquid nitrogen tanks are installed require sufficient ventilation to maintain an inflow of breathable air for the safety of the employees, which bears additional costs on infrastructure and electricity consumption.

4/ uniformity of temperature inside the Dewar’s vessels, in which biological samples are stored, is not as good as the producers of such systems claim. According to Loyd Wolfinbarger (1998), the temperatures in such vessels can reach up to -70ºC, which is absolutely unacceptable from the point of view of sample integrity.

In contrast to the standard systems, Mirai Cryo, cryogenic refrigeration system that works with nitrogen as the refrigerant, is reliable, safe and requires only electricity to operate and keep your samples in top condition.

Mirai Cryo is suitable for providing cooling to a closed chamber, maintaining a temperature down to – 160ºC with the accuracy of +/- 0,5 °C, is efficient, environmental, and most importantly safe to humans.

SUITABLE FOR APPLICATIONS:

• Umbilical cord blood as a source of hematopoietic stem and progenitor cells
• Stem cells for autologous transplants in patients who have undergone high dose chemotherapy
• Adipose tissue, epithelial cells and bone marrow for stem cell therapy
• Blood products for immunology analysis
• Mesenchymal stromal cells for regenerative medicine and tissue engineering
• Cancerous tissue samples
• Semen for artificial insemination – used for breeding guide dogs and race horses
• Oocytes and embryos for IVF
• Ovarian tissue for preserved reproductive function in women undergoing treatments
• Plant seeds/shoots for breeding
• Components for shrink fitting of parts in industrial manufacturing
• Temperature testing of parts in the automotive and aerospace industry